Coding

Part:BBa_K3628005:Design

Designed by: Tianzuo Zeng   Group: iGEM20_SMS_Shenzhen   (2020-10-27)


pMag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 178
    Illegal AgeI site found at 406
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use this to switch the activation of T7 RNA Polymerase.


Source

We artificially synthesize it Kawano, F., Suzuki, H., Furuya, A. and Sato, M., 2015. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nature Communications, 6(1).

References